Description: Ascorbic acid is a vital water-soluble antioxidant found in living organisms. Ascorbic acid is critical for a variety of functions related to tissue growth and wound healing, neurotransmitter formation, blood cholesterol levels, as well as free radical neutralization. The OxiSelect Ascorbic Acid Assay Kit is a quantitative assay for measuring ascorbic acid within various samples. The assay is based on the Ferric Reducing/Antioxidant Ascorbic Acid (FRASC) chemistry driven by the electron donating reducing power of antioxidants. The kit employs ascorbate oxidase, which allows the user to differentiate the ascorbic acid content from other antioxidants present within their sample. Ascorbic acid levels in a sample are determined by measuring the difference in optical density between two sample wells, one with and one without the enzyme.

Description: Our Monoamine Oxidase Assay Kit measures MOA-A and MAO-B in biological samples. MAO reacts with a substrate, generating hydrogen peroxide. A reaction between hydrogen peroxide and the probe results in a signal that is directly proportional to the level of MAO in the sample and is detected fluorescence plate reader. MAO activity in unknown samples is calculated based on a hydrogen peroxide standard curve. 

Description: Our Monoamine Oxidase Assay Kit measures MOA-A and MAO-B in biological samples. MAO reacts with a substrate, generating hydrogen peroxide. A reaction between hydrogen peroxide and the probe results in a signal that is directly proportional to the level of MAO in the sample and is detected fluorescence plate reader. MAO activity in unknown samples is calculated based on a hydrogen peroxide standard curve. 

Description: Our Monoamine Oxidase Assay Kit measures MOA-A and MAO-B in biological samples. MAO reacts with a substrate, generating hydrogen peroxide. A reaction between hydrogen peroxide and the probe results in a signal that is directly proportional to the level of MAO in the sample and is detected colorimetric plate reader. MAO activity in unknown samples is calculated based on a hydrogen peroxide standard curve. 

Description: Our Monoamine Oxidase Assay Kit measures MOA-A and MAO-B in biological samples. MAO reacts with a substrate, generating hydrogen peroxide. A reaction between hydrogen peroxide and the probe results in a signal that is directly proportional to the level of MAO in the sample and is detected colorimetric plate reader. MAO activity in unknown samples is calculated based on a hydrogen peroxide standard curve. 

Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl Fluorometric Assay Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The fluorescence plate-based format provides a convenient system for direct measurement of protein carbonyl content.

Description: The OxiSelect Total Glutathione Assay Kit is a quantitative assay for measuring the total glutathione content within a sample (GSH/GSSG).  Glutathione Reductase reduces oxidized glutathione (GSSG) to reduced glutathione (GSH) in the presence of NADPH.  Subsequently, the  chromogen reacts with the thiol group of GSH to produce a colored compound that absorbs at 405 nm.  The total glutathione content in unknown samples is determined by comparison with the predetermined glutathione standard curve.  The rate of chromophore production is proportional to the concentration of glutathione within the sample.  The rate can be determined from the absorbance change over time.  Metaphosphoric acid is provided to remove interfering proteins or enzymes from samples.

Description: Superoxide dismutase (SOD), which catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen, is one of the most important antioxidant enzymes. SOD enzymes are classified into three groups: cytosolic CuZn-SOD, mitochondrial Mn-SOD, and extracellular Ec-SOD. Our OxiSelect Superoxide Dismutase Activity Assay uses a xanthine/xanthine oxidase (XOD) system to generate superoxide anions and a chromagen to produce a water-soluble formazan dye upon reduction by superoxide anions. The superoxide dismutase activity is determined as the inhibition or reduction of chromagen.

Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

Description: The OxiSelect Hydrogen Peroxide/Peroxidase Assay Kit is a sensitive quantitative fluorometric assay for hydrogen peroxide or peroxidase.   In the presence of HRP, ADHP reacts with H2O2 in a 1:1 stoichiometry to produce highly fluorescent Resorufin.  The Resorufin product can be easily read by a fluorescence microplate reader with an excitation of 530-560 nm and an emission of 590 nm, or for absorbance at 560 nm.  Fluorescence values are proportional to the H2O2 or peroxidase levels within the samples.  The H2O2 or peroxidase content in unknown samples is determined by comparison with its respective standard curve.

Description: The OxiSelect Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells.  Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested.  After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator.  The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF.  The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.

Description: Our OxiSelect 3-Well Comet Assay Kits provide a fast, convenient way to screen for general DNA damage, regardless of the source or nature of the damage. Kits include Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

Description: Our OxiSelect 3-Well Comet Assay Kits provide a fast, convenient way to screen for general DNA damage, regardless of the source or nature of the damage. Kits include Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

Description: Our OxiSelect 3-Well Comet Assay Kits provide a fast, convenient way to screen for general DNA damage, regardless of the source or nature of the damage. Kits include Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

Description: The OxiSelect Total Antioxidant Capacity (TAC) Assay measures the total antioxidant capacity of biomolecules from a variety of samples via a SET mechanism. In the presence of antioxidants, copper(II) is reduced to copper(I). In turn, the copper(I) ions react with a chromogen to produce a color with maximum absorbance at 490nm.

Description: Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity. The OxiSelect Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, colorimetric assay for measuring the myeloperoxidase activity within a sample.